WIDE FIELD FLUORESCENCE MICROSCOPE
The Zeiss Axio Imager M2 interacts with ZEN for image capture and processing. ZEN has a wealth of general functions for image optimization and documentation with the following additional modules:
·Multichannel Acquisition software controls the motorized reflector turret allowing for rapid automatic acquisition of multiple channels with optimal exposure times for each channel.
·Interactive Measurement for accurate measurement of distances, areas, and pixel intensity.
·Z-Stack module controls the Z-drive of the motorized stage for Z-stack generation and orthogonal analysis.
·3D Deconvolution for computational reduction of out-of-focus fluorescence in three-dimensional microscope images resulting in images with less noise, better definition, and higher resolution.
Zeiss Imager Fluorescence Microscope
MICROSCOPE AND AXIOVISION $25/hr
All users must go thru a training/orientation session prior to using any of the instrumentation.
Please contact Bryan Anthony for information or scheduling orientation/training sessions.
Zeiss Axio Imager M2 Microscope (upright)
The refelector turret is automated allowing for automatic acquisition of multichannel images with ZEN software.
The stage is automated for precise Z-step acquisition for Z-stack analysis and 3D Deconvolution with ZEN software.
10X, 20X, 40X oil (phase and DIC), 63X oil (phase and DIC), and 100X oil (phase and DIC).
DAPI, GFP, DsRed, and Cy5.
The system is equipped with an Axiocam MRm Monochrome Camera with the following features:
·High 12-bit dynamics
·Maximum resolution in 1300 X 1030 pixels ·Expanded spectral range
·High sensitivity for near infrared
FINAL IMAGES All images will be archived in czi format which can be viewed with ZEN software.
You may also view these on any PC using ZEN lite which can be downloaded free from the Zeiss website.
Most users export their images as tif or jpg files or as avi or mov for movie formats.